Effects of both high glucose and high insulin on proliferations and migrations of human vascular smooth muscle cells as well as miR-145 level
- VernacularTitle:高糖及高胰岛素对人血管平滑肌细胞增殖、迁移及miR-145水平的影响
- Author:
Chengji JIN
;
Tian TANG
;
Xiaojing YU
;
Jianmei QU
;
Wei DUAN
;
Tao ZHANG
- Keywords:
miR-145;
vascular smooth muscle cells;
diabetes mellitus
- From:
Basic & Clinical Medicine
2017;37(1):94-97
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the effect of both high glucose and high insulin on miR-145 level.Methods The human vascular smooth muscle cells ( VSMCs) were cultured and the proliferation of VSMCs was induced by high glucose and high insulin medium.The samples were divided into 4 groups: normal group, high glu-cose group (25 mmol/L), high insulin group (300 mU/L), high glucose and high insulin group.The ex-pression of miR-145 in VSMCs was assayed by real-time PCR.Proliferation of VSMCs was determined by MTT method After 72 h cultivation.The migration of VSMCs was analyzed by cell scratch test .VSMCs in each group was transfected by miR-145 virus ( lentiviral vector ) .Proliferation and migration were assayed after 48 h transfection.Results The expression of miR-145 in VSMCs of other three groups was decreased ( P<0.05 ) , especially the expression in the high glucose and insulin group was the lowest ( P<0.01 ) .Prolifera-tion and migration of VSMCs was promoted by high glucose and/or high insulin medium.Under fluorescent, transfection rate of VSMCs was about 80%after 48 h transfection.Proliferation and migration of VSMCs in each group after transfection were significantly lower than before ( P<0.05 ) .Conclusions High glucose and high in-sulin could decrease the expression of miR-145 in VSMCs, The overexpression of miR-145 may inhibit the prolif-eration and migration of VSMCs .
