Expressions of miR-21 and SnoN in kidney of diabetic rats
- VernacularTitle:糖尿病大鼠肾组织中miR-21和SnoN表达的变化
- Author:
Lingling LIU
;
Lirong LIU
;
Yuanyuan WANG
;
Mingjun SHI
;
Ying XIAO
;
Yingying ZHANG
;
Xiaohuan ZHANG
;
Bing GUO
- Keywords:
diabetic nephropathy;
Ski-related novel protein N(SnoN) protein;
p-Smad3(Ser423/425) protein;
miR-21
- From:
Basic & Clinical Medicine
2017;37(1):32-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and possible mechanism of miR-21 and Ski-related novel protein N( SnoN) in the renal fibrosis diabetic process.Methods The animal model was established by tail-vein injection of Streptozotocin,and the other group were normal control ( NC) group.After 10 weeks, the rats were sacrificed to measure biochemical parameters and renal index , and to observe the changes of pathomorphology by HE staining as well.Meanwhile, immunohistochemistry and Western blot were employed to examine protein ex-pression of E-cadherin,α-smooth muscle actin(α-SMA), fibronectin(FN), collagen-Ⅰ(Col-Ⅰ), collagen-Ⅲ(Col-Ⅲ), transforming growth factor-β1(TGF-β1), Smad3, p-Smad3(Ser423/425) and SnoN in the renal tissue. In addition, the expression of SonN mRNA and miR-21 were detected by qPCR.Results In DM group,the ex-pressions of Col-Ⅰ, Col-Ⅲ and FN in renal interstitium were increased ( P <0.05 ) , TGF-β1 increased (P<0.05),while E-cadherin decreased(P<0.05).Compared with NC group, the expression of α-SMA,p-Smad3 (Ser423/425) protein increased in DM group(P<0.05),while the protein level of SnoN decreased but the level of SnoN mRNA increased ( P <0.05 ) .Moreover, the level of miR-21 markedly increased in DM group ( P <0.05 ) .Conclusions TGF-β1 may up-regulate the expression of miR-21 but restrain the translational expression of SnoN, aggravating fibrosis.
