Effects of miRNA-181a on proliferation and migration of multiple myelo-ma cells
10.3969/j.issn.1000-4718.2017.01.006
- VernacularTitle:miRNA－181a 对多发性骨髓瘤细胞增殖和迁移的作用
- Author:
Xiao YAN
;
Yanli ZHANG
;
Guifang OUYANG
;
Qitian MU
;
Lixia SHENG
- Keywords:
miRNA-181a;
Multiple myeloma;
Cell cycle
- From:
Chinese Journal of Pathophysiology
2017;33(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effect of miRNA-181a inhibition on the proliferation, migration and cell cycle of the human multiple myeloma cell line RPMI 8226.METHODS:Real-time PCR was used to detect miRNA-181a expression in serum samples from multiple myeloma or healthy subjects .After transfection with miRNA-181a inhibitor, the cell viability was examined by CCK-8 assay and colony formation assay .The cell migration ability was analyzed by wound healing assay .The cell cycle was detected by flow cytometry .Moreover , the protein level of cyclin D 1 and the phosphoryla-tion of PI3K and Akt were determined by Western blot .RESULTS:The expression of miRNA-181a was significantly in-creased in the serum from multiple myeloma patients as compared with healthy group .Inhibition of miRNA-181a expression by transfection with miRNA-181a inhibitor remarkably decreased the cell viability , migratory ability, the population of G0/G1 phase and cyclin D1 protein expression in the RPMI8226 cells.However, the population of S phase and the phosphory-lation of PI3K and Akt were reduced .CONCLUSION:Down-regulation of miRNA-181a inhibits the viability and migra-tory ability in the RPMI8226 cells via inhibition of cell cycle and PI 3K/Akt signaling pathway .
