Effects of tonifying kidney yin and yang on expressions of TNF-α, IFN-γin peripheral blood and apoptosis of granulosa cells in chemotherapy-in-duced premature ovarian failure rats
10.3969/j.issn.1000-4718.2017.01.023
- VernacularTitle:补肾阴及补肾阳法对化疗诱导的卵巢早衰大鼠外周血TNF－α、IFN－γ水平及卵巢颗粒细胞凋亡的影响
- Author:
Jiali LIANG
;
Sioman KUONG
;
Jing YANG
;
Jiajia QIN
- Keywords:
Premature ovarian failure;
Method of tonifying the kidney yin;
Method of tonifying the kidney yang;
Chemotherapy
- From:
Chinese Journal of Pathophysiology
2017;33(1):139-142
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To treat the chemotherapy-induced premature ovarian failure ( POF) rats with the methods of tonifying kidney yin and yang by observing the indicator changes in apoptosis of ovarian granulosa cells and related cyto -kines before and after medication .METHODS:SD rats ( n=36) were chronically given intraperitoneal injection of cyclo-phosphamide ( CTX) for 15 d in order to establish the animal model of POF .The rats were randomly divided into control group, CTX group, CTX+kidney yin group and CTX +kidney yang group.The levels of tumor necrosis factor alpha (TNF-α) and interferon gamma ( IFN-γ) in the peripheral blood were detected by ELISA .The apoptosis of ovarian granulosa cells was observed by TUNEL method .The levels of apoptosis related proteins were determined by Western blot .RE-SULTS:After modeling , the levels of TNF-αand IFN-γin the peripheral blood were significantly increased .The protein expression of Bax was up-regulated and Bcl-2 was down-regulated .The apoptosis of ovarian granulosa cells was significantly induced with a deep staining .After treatment with tonifying kidney yin and yang , the levels of TNF-αand IFN-γin the pe-ripheral blood were decreased , the protein expression of Bax was down-regulated and Bcl-2 was up-regulated, and the apop-tosis of ovarian granulosa cells was reduced .CONCLUSION:The reserve function of the ovarian can be recovered by pre-scriptions of tonifying the kidney yin and yang via regulating the expression of TNF -α, IFN-γ, Bax and Bcl-2, and inhibi-ting the apoptosis of ovarian granulosa cells .
