Platycodin D protects oral epithelial cells against infection of Candida albi-cans
10.3969/j.issn.1000-4718.2017.01.027
- VernacularTitle:桔梗皂苷D防御口腔黏膜上皮细胞感染白色念珠菌的作用
- Author:
Lifen ZHU
;
Bing WANG
- Keywords:
Platycodin D;
Oral epithelial cells;
Candida albicans
- From:
Chinese Journal of Pathophysiology
2017;33(1):161-165
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the effect of platycodin D on Candida albicans infection in oral epithelial cells.METHODS:The viability of the oral squamous carcinoma KB cells was detected by MTT assay after treated with different concentrations of platycodin D .The KB cells were infected with Candida albicans, and then were incubated with platycodin D at different concentrations .Adherent numbers of the Candida albicans were counted by Gram staining , and the bacterial activity and conversion were measured by Trypan blue staining .Furthermore , the protein levels of IL-18 and hu-man β-defensin 2 ( HBD-2) were analyzed by ELISA , and the expression of HBD-2 at mRNA and protein levels was deter-mined by RT-qPCR and Western blot , respectively .RESULTS:The viability of the KB cells was not affected by platycod-in D at the concentrations used .The adherent numbers , bacterial activity and conversion were decreased by treatment with platycodin D in a dose-dependent manner .In addition, the protein level of IL-18 in the culture supernatant and the mRNA expression of HBD-2 in the KB cells were also reduced after platycodin D treatment .CONCLUSION:Platycodin D has a bacteriostasis effect and prevents oral epithelial cells from Candida albicans infection.
