Effects of BCL6 B on proliferation and migration of human colorectal car-cinoma LoVo cells and its potential mechanism
10.3969/j.issn.1000-4718.2017.01.007
- VernacularTitle:BCL6B对人结直肠癌LoVo细胞增殖和迁移的影响及机制
- Author:
Yue GU
;
Aifang LI
;
Hui SUN
;
Xueru LI
;
He ZHA
;
Jiali ZHAO
;
Jiaqing XIE
;
Lan ZHOU
- Keywords:
BCL6B;
Colorectal cancer;
Cell proliferation;
Cell migration;
PI3K/AKT signaling pathway
- From:
Chinese Journal of Pathophysiology
2017;33(1):38-45
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To detect the endogenous expression of B-cell leukemia/lymphoma 6 member B (BCL6B) in FHC and LoVo cells, and to investigate the effects of BCL6B on proliferation and migration of LoVo cells for further explo-ring the underlying mechanism .METHODS:The endogenous expression of BCL 6B in the FHC and LoVo cells was detec-ted by RT-PCR and Western blot .The methods of MTT assay , colony formation assay , wound healing assay and Transwell chamber experiment were employed to examine the biological functions of BCL 6B in the LoVo cells.The mRNA and protein levels of BCL6B, cyclin D1 and matrix metalloproteinase-9 ( MMP-9) were determined by RT-PCR and Western blot , re-spectively.The level of phosphorylated protein kinase B (p-AKT) was detected by Western blot.RESULTS:BCL6B ex-pression was notably repressed in the LoVo cells as compared with the FHC cells , which were significantly increased by transfection with pcDNA3.1-BCL6B.The abilities of proliferation and migration of the LoVo cells at 72 h were inhibited by 28.33%(P<0.01) and 36.11%(P<0.05) in BCL6B group.The mRNA levels of cyclin D1 and MMP-9 in the cells of BCL6B group were decreased by 39.90%(P<0.01) and 77.36% (P <0.05), and the protein levels of cyclin D1, MMP-9 and p-AKT were reduced by 44.00%(P<0.05), 47.06%(P<0.01) and 32.88% (P<0.05), respectively. CONCLUSION:BCL6B inhibits proliferation and migration of the LoVo cells , and the PI3K/AKT signaling pathway is in-volved in this process .
