Use of Immunochromatographic Assays for Identification of Mycobacterium tuberculosis Complex from Broth Cultures.
- Author:
Young Kyung LEE
1
;
Han Sung KIM
;
Wonkeun SONG
;
Jae Seok KIM
;
Hee Jung KANG
;
Kyu Man LEE
Author Information
1. Department of Laboratory Medicine, Hallym University College of Medicine, Chuncheon, Korea. kimhan@hallym.ac.kr
- Publication Type:Original Article
- Keywords:
Mycobacterium tuberculosis complex;
Immunochromatographic assay;
Identification
- MeSH:
Immunochromatography;
Mycobacterium;
Mycobacterium tuberculosis;
Real-Time Polymerase Chain Reaction
- From:Journal of Laboratory Medicine and Quality Assurance
2011;33(1):39-42
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Broth cultures are increasingly used to detect acid-fast bacilli (AFB). Rapid, simple and accurate methods for differentiation of Mycobacterium tuberculosis complex (MTBC) and nontuberculosis mycobacteria from broth cultures are needed. Immunochromatographic assays (ICTs) for identification of MTBC have been developed. METHODS: The abilities of the BD MGIT TBc Identification Test (Becton Dickinson, USA) and the SD Bioline TB Ag MPT64 (Standard Diagnostics, Korea) to detect MTBC were evaluated in 44 AFB-positive broth cultures. The results of 2 ICTs were compared to those of real-time PCR. RESULTS: The BD MGIT TBc Identification Test and the SD Bioline TB Ag MPT64 showed concordant results with real-time PCR by 100% and 97.7%, respectively. The sensitivity of the BD MGIT TBc Identification and the SD Bioline TB Ag MPT64 was 100% for both, and the specificities of those were 100% and 95.2%, respectively. CONCLUSIONS: Both ICTs are rapid methods for identification of MTBC from broth cultures, and the results of ICTs are in accord with those of real-time PCR.
