Evaluation of two Real-Time PCR Assays for the Detection of Chlamydia trachomatis.
- Author:
Eun Young LEE
1
;
Sinyoung KIM
;
Hyon Suk KIM
Author Information
1. Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. sykim@yuhs.ac
- Publication Type:Original Article
- Keywords:
Chlamydia trachomatis;
Real-time polymerase chain reaction;
Comparison
- MeSH:
Biological Science Disciplines;
Chlamydia;
Chlamydia trachomatis;
Humans;
Real-Time Polymerase Chain Reaction
- From:Journal of Laboratory Medicine and Quality Assurance
2011;33(1):43-47
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Chlamydia trachomatis is common etiological agent of sexual transmitted disease. We evaluated two real-time PCR assays for the detection of C. trachomatis; Cobas TaqMan CT test v2.0 (Roche Diagnostics, Switzerland) and AdvanSure CT/NG real-time PCR (LG Life Sciences, Korea). And we compared performance of two real-time PCR assays with Cobas Amplicor CT test (Roche Diagnostics, Switzerland). METHODS: Seventy-nine cervical swab specimens collected from patients with suspected C. trachomatis infection were tested using CTM CT, ADVANSURE, and AMPLICOR for the detection of C. trachomatis. RESULTS: Twenty-five (31.6%) samples showed positive results in all three assays. The positive and negative concordance rates among three assays were 100%. CONCLUSIONS: CTM CT and ADVANSURE showed excellent agreement with AMPLICOR. Therefore, these assays were reliable for the evaluation of C. trachomatis infection.
