The application of ultraifltration in old biological samples(
10.13618/j.issn.1001-5728.2016.06.020
- VernacularTitle:超滤管在陈旧生物检材中的应用
- Author:
Jianli GU
;
Yanni LIAO
;
Ting LIANG
;
Yan WANG
;
Qin ZENG
- Keywords:
forensic biological evidence;
ultraifltration device;
old biological samples;
DNA typing
- From:
Chinese Journal of Forensic Medicine
2016;31(6):607-609
- CountryChina
- Language:Chinese
-
Abstract:
Objectve To investigate the application value of ultraifltration with millipore ifltration method test of old biological samples DNA. Methods 23 old blood samples separately were cut blood piece equally suitable size of three groups, labeled A, B and C group. DNA was extracted by magnetic bead methed and get DNA template with 80μL, 80μL, 20μL elution solution, respectively, then the DNA template in group A and C take right amount directly ampliifed, group B with milipore ultraifltration ifltration condensed amplication. PCR products were detected by ABI3130xl genetic analyzer and analyzed by GeneMapperID V3.2 software. The date were analyzed by using SPSS software. Results No samples were ampliifed for all STR loci in group A. There were 18 cases in group B samples amplified all STR loci and 11 samples in group C. Conclusion Application of millipore ultrafiltration method can signiifcantly improve the old biological samples DNA classiifcation success rate.
