The expression of basigin mRNA in early ischemic myocardium of rats
10.13618/j.issn.1001-5728.2016.06.003
- VernacularTitle:大鼠早期缺血心肌中Basigin mRNA的表达
- Author:
Zheng LIANG
;
Zhiqiang WEN
;
Junyuan QIAO
;
Gengqian ZHANG
- Keywords:
forensic pathology;
basigin;
real-time PCR;
early myocardial ischemia
- From:
Chinese Journal of Forensic Medicine
2016;31(6):547-549
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression differences of Basigin(BSG)mRNA between early ischemic myocardium(EIM) and non-ischemic myocardium(NIM) in rats and then to evaluate the possibility of BSG examination in ischemic myocardium accidents occurred in forensic medicine. Methods Real-time polymerase chain reaction (RT-PCR) technique was applied for detecting the expression of BSG mRNA in EIM and NIM of rats at 15min, 30min, 1h and 2h post myocardial ischemia, and in sham operation(SO) group. Results Compared with NIM, SO and control groups, expression of BSG mRNA decreased after 15min when myocardium ischemia occurred; compared with SO, it was of 0.5 folds when 1h(P<0.5), and rebounded to SO level when 2h. Conclusion BSG could be involved in protection and myocardial remodeling in early ischemic myocardium, and may serves as a biomarker of early ischemic myocardium.
