Establishment of quality standard for Tibetan medicineRuyi-Zhenbao pill

VernacularTitle:藏药如意珍宝丸的质量标准研究
Author: Yanan HAN ; Ming YUAN ; Juan ZHOU ; Juan WANG
Keywords: Chromatography,thin layer; Chromatography,High pressure liquid; Quality control; Ruyi-Zhenbao pill
From: International Journal of Traditional Chinese Medicine 2017;39(1):53-58
CountryChina
Language:Chinese
Abstract: Objective To establish a quality standard for Tibetan medicineRuyi-Zhenbao pill. Methods Thin-layer chromatography (TLC) was applied to identify 12 Tibetan medicinal materials, and High performance liquid chromatography (HPLC) was employed to determine the contents of gallic acid, dehydrodiisoeugenol, piperine, and cinnamaldehyde.Results The identified characteristics of TLC were distinct and the spots were clear. Linearity of gallic acid, dehydrodiisoeugenol, piperine, and cinnamaldehyde were in the range of 0.106-0.901μg, 0.033-0.281μg, 0.007-0.060μg, and 0.021-0.178μg, respectively. Average recovery was in the range of 98.47%-101.65% (RSD<3.0%).Conclusions The method of identification and content determination was good in terms of specificity, accuracy and repeatability, and can be used for quality control ofRuyi-Zhenbaopill.