Study on the TLC and quantitative determination by UV-HPLC forBaiduyinsyrup

VernacularTitle:败毒饮的薄层鉴别与含量测定
Author: Xiaoke HAN ; Chaofeng LIANG ; Jun QI
Keywords: Baicalin; Buddleoside; Chromatography,thin layer; Chromatography,high pressure liquid; Quality control; Baidusyrup
From: International Journal of Traditional Chinese Medicine 2017;39(1):59-62
CountryChina
Language:Chinese
Abstract: Objective To establish the quality control ofBaiduyin syrup.Methods The TLC was used to identity Radix paeoniae rubra, Radix Scutellariae. The quantitative determination of baicalin and buddleoside was completed by HPLC.Results The spots on TLC plates were distinct and high resolution. Compared with the negative samples, the contrast medicinal materials or control products showed that there were no spots of the same color in the corresponding position. The linear ranges of baicalin and buddleoside were 0.2179-2.1790 μg (r2=0.999 9), 0.1319-1.3190 μg (r2=0.999 7). TheRSD were 1.51% and 2.01%. Conclusions The established quality control method is simple, accurate and reproducible, which can be used for the quality control ofBaiduyin syrup.