SCCmec genotyping and antimicrobial susceptibility of community-ac-quired methicillin-resistant Staphylococcus aureus
10.3969/j.issn.1671-9638.2016.12.001
- VernacularTitle:社区获得性耐甲氧西林金黄色葡萄球菌SCCmec基因分型及药敏结果
- Author:
Xingang WANG
;
Baosheng DUAN
- Keywords:
Staphylococcus aureus;
community-acquired methicillin-resistant Staphylococcus aureus;
CA-MR-SA;
SCCmec genotyping;
drug resistance,microbial;
rational antimicrobial use
- From:
Chinese Journal of Infection Control
2016;15(12):897-901
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the types of staphylococcal cassette chromosome mec (SCCmec)gene and an-timicrobial resistance of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA)isolated from outpatients and inpatients in a hospital.Methods MRSA strains isolated between May 2011 and August 2015 in a hospi-tal and the relevant case data were collected,polymerase chain reaction(PCR)method was used to identify mecA gene of MRSA and SCCmec gene of CA-MRSA,antimicrobial susceptibility testing of CA-MRSA were performed and analyzed. Results A total of 305 MRSA isolates were collected,296 of which were mecA positive,29.73% (88/296)were CA-MR-SA. The genotyping of CA-MRSA showed that 48 strains were SCCmec type Ⅳ,36 were SCCmec type V,the other 4 strains were undefined. Antimicrobial susceptibility testing results showed that susceptibility rates of CA-MRSA to vanco-mycin,linezolid,and tigecycline were all 100% ,resistance rates to penicillin and oxacillin were both 100% ;resistance rates of SCCmec type IV and SCCmec type V CA-MRSA strains to levofloxacin,rifampicin,and ciprofloxacin were all signifi-cantly different (all P<0.05),to ampicillin/sulbactam,furantoin,and erythromycin were all >58% .Conclusion The main SCCmec type of CA-MRSA are type IV and type V in this hospital,antimicrobial resistance rate is high,clinicians should pay high attention,and use antimicrobial agents according to antimicrobial susceptibility testing results.
