Expression of TLR4 in monocytes and its correlation with TNF-α and IL-6 in patients with diabetic neuropathy
10.3969/j.issn.1006-6187.2017.02.001
- VernacularTitle:糖尿病神经病变患者单核细胞 TOLL 样受体4的表达及其与血浆肿瘤坏死因子α、白介素6的相关性研究
- Author:
Qingsheng MENG
;
Tao ZHU
- Keywords:
Diabetic neuropathy;
TLR4;
Monocytes;
TNF-α;
IL-6
- From:Chinese Journal of Diabetes
2017;25(2):97-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Toll-like receptor 4 (TLR4)in peripheral blood monocytes and its correlation with TNF-α and IL-6 in type 2 diabetes (T2DM)patients with neuropathy. Methods Healthy volunteers (NC,n=44),T2DM without neuropathy (n=44)and T2DM with neuropathy (n=44)were recruited in the present study.The expression of TLR4 mRNA and protein in peripheral blood monocytes was detected by RT-PCR and flow cytometry. ELISA was undertaken to measure the plasma concentration of TNF-αand IL-6. Results The levels of HbA1 c[(9.09±1.62)%vs(8.36±1.10)% vs (5.30±0.89)%],the protein expression of TLR4[(42.02±9.69)% vs (31.27± 6.87)% vs (11.96±5.54)%],the mRNA expression of TLR4[(2.98±1.06)vs (1.74±0.47)vs (1.12 ±0.52)],TNF-α[(8.75±3.14 )vs (6.27±3.64)vs(3.19±1.17)pg/ml]and IL-6[(3.63±1.81)vs(2.60 ±1.14)vs(1.54±0.58)pg/ml]in patients with diabetic neuropathy significantly increased compared with T2DM and NC group;TLR4 showed a positive correlation with TNF-αand IL-6(r=0.631,P <0.0001;r=0.447,P =0.0023). Conclusion TLR4 of monocytes may take part in the occurrence of systemic inflammation and has a close relationship with the development of diabetic neuropathy.
