Expression,purification and bioimformatics analysis of allergen protein Profilin from silkworm (Bombyx mori)
10.3969/j.issn.1000-484X.2017.01.016
- VernacularTitle:家蚕过敏原Profilin的表达、纯化、免疫学鉴定及其生物信息学分析
- Author:
Wei HU
;
Zhilin LIANG
;
Lianglu WANG
;
Huiling ZHONG
;
Zhigang LIU
- Keywords:
Silkworm;
Profilin protein;
Allergen
- From:
Chinese Journal of Immunology
2017;33(1):81-84
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain recombinant Profilin of silkworm,identify its immunogenicity,predict its B cell epitopes and construct the evolutionary trees. Methods: The nucleotide sequence of Profilin was acquired from NCBI,synthesized it and cloned it into pET-28 vector. Then,the recombinant plasimids were transformed to E. coli BL21. After induced by IPTG,recombinant protein was purified by Affinity chromatography. Furtherly,its allergenicity was identified by Western blot,the potential B cell epitopes was analyzed through DNAStar and build the evolutionary trees by MEGA5. 05. Results: The recombinant protein of Profilin was successfully expressed and purified by affinity chromatography. Besides,the protein contains a high IgE-binding activity with IgE existing in serum of patients allergic to silkworm. Conclusion: The recombinant proflilin has IgE-binding activity, and it is meaningful for fundamental research and specific diagnosis studies of allergic diseases caused by silkworm.
