Mechanisms of peripheral basophils activation in patients with rheumatoid ar-thritis
10.3969/j.issn.1000-484X.2017.01.017
- VernacularTitle:类风湿性关节炎患者嗜碱性粒细胞活化机制研究
- Author:
Qiaofen LAN
;
Huanjin LIAO
;
Qiuhua CHEN
;
Jun CAI
;
Lifang ZHANG
;
Yanxia PENG
;
Yiming HE
;
Ping WU
;
Tong XIE
;
Qingjun PAN
- Keywords:
Rheumatoid arthritis;
Basophil;
Activation;
IgE;
IL-4
- From:
Chinese Journal of Immunology
2017;33(1):85-89
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the activation of peripheral basophils from patients with rheumatoid arthritis ( RA) and its mechanisms. Methods:The activation markers including CD203c and the proportion of IL-4 positive rate of peripheral basophils from RA patients and healthy controls were detected by flow cytometry. Serum levels of IgE in RA patients and healthy controls were detected by electrochemilu minescence immunoassay. Basophils were negatively isolated and co-cultured with or without purified IgE,anti-IgE as positive control,then,the expression of CD203c and propotion of IL-4 positive basophils were detected by flow cytometry. Results:The expression of CD203c and IL-4 positive rate of basophils from RA patients were higher than that of healthy controls (P<0. 05). Serum levels of IgE in RA were higher than that of healthy controls (P<0. 05). After co-cultured with isolated IgE from RA patients,basophils negatively isolated from healthy controls were activated,and higher expression of CD203c and proportion of IL-4 (P<0.05). Conclusion:Basophil activation is related with development of RA,and its activation is mainly mediated by IgE. Targeting basophil and its activation pathway would be expected to provide new strategies for the treatment of RA.
