Mechanism of thiocoraline combination with cisplatin on promoting apoptosis of cervical cancer cell line c4-1 and Hela
10.3969/j.issn.1000-484X.2017.01.020
- VernacularTitle:噻可拉林协同顺铂促进宫颈癌c4-1及Hela细胞凋亡机制的研究
- Author:
Hongling DONG
;
Ping ZHOU
;
Wenjing YANG
- Keywords:
Thiocoraline;
Cisplatin;
Cervical carcinoma;
Apoptosis
- From:
Chinese Journal of Immunology
2017;33(1):99-102
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the apoptosis by thiocoraline in combination with cisplatin on cervical cell line c4-1 and Hela and its mechanism. Methods:Different concentrations of thiocoraline and cisplatin alone or in combination used for cultured c4-1 and Hela cells. The inhibition of cell proliferation was detected by MTT assay,and the apoptosis was detected by Hoechst 33342 and AO-EB staining. The expressions of the protein Bax,Bcl2,p53,p21 were detected by Western blot. The cell cycle of c4-1 and Hela cells were detected by Flow cytometry. Results: The cell proliferation activity of c4-1 and HeLa cells were inhibited obviously by different concentrations of thiocoraline and cisplatin alone or in combination. The inhibition effect was in a dose-dependent manner. Meanwhile, when the concentrations of thiocoraline and cisplatin were 10 nmol/L and 4 μmol/L, respectivity the inhibitory effect was most significant (P<0. 05);Hoechst 33342 and AO-EB staining showed that the cell apoptosis effect was more obvious on thiocoraline in combination with cisplatin. The Western blot showed that the expressions of the protein Bax,p53,p21 were significantly up-regulated, and the Bcl2 was significantly down-regulated. Conclusion:Thiocoraline combination with cisplatin could up-regulate the expression of the protein Bax,p53,p21 and down-regulation the expression of Bcl2 to inhibition the proliferation and promotion apoptosis of cervical cell line c4-1 and Hela.
