Therapeutical effect of Irbesartan for coronary atherosclerosis mice (ApoE-/-) and its possible mechanisms
10.3969/j.issn.1000-484X.2017.01.026
- VernacularTitle:Irbesartan对冠状动脉粥样硬化小鼠的治疗作用及可能机制
- Author:
Yi YU
;
Zengqi LI
;
Jian LIAO
- Keywords:
Irbesartan;
Animal model;
Coronary atherosclerotic heart disease;
Adipose tissue;
Inflammation
- From:
Chinese Journal of Immunology
2017;33(1):126-129
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the therapeutical effect of Irbesartan for coronary atherosclerosis mice ( ApoE-/-) and its possible mechanisms. Methods: A total of 16 male ApoE-/- mice were randomly divided into control group and Treatment group [Irbesartan,50 mg/(kg·d),4 weeks]. HE,immunofluorescence,Western blot and ELISA were performed to analyze the effect of Irbesartan on ApoE-/- and the changes of related signaling pathways. Results: Compared with control group,the treatment group had lower atheroma macular areas and inflammatory cytokines in blood vessel (P<0. 05). Treatment group had lower levels of leptin,but higher levels of PPAR-γ and adiponectin in perivascular adipose tissues ( PVAT ) than these of control group, the difference were statistically significant ( P<0. 05 ) . Western blot and immunofluorescence analysis shown that Irbesartan treatment significantly depressed the expression of p-p65 and p-IKK in PVAT when compared with these of control group (P<0. 05). Conclusion:Irbesartan has significantly therapeutic effect on ApoE-/-mice,the possible mechanisms including anti-inflammatory effects in PVAT,improved the adipose tissue function and regulated the PPAR-γ-NF-κB signaling pathways.
