A comparative study of two reprogramming systems for inducing pluripotent stem cells from human dental origin
10.3969/j.issn.1671-8348.2017.01.021
- VernacularTitle:两种重编程系统诱导人牙源性多潜能干细胞的对比研究
- Author:
Xiaobing TAN
;
Jingshu XU
;
Guihu SUN
;
Juncheng SONG
;
Qingyuan DAI
- Keywords:
pluripotent stem cells;
nipples;
stem cells;
lentivirus;
human induced pluripotent stem cells;
stem cells from apical papilla;
reprogramming;
sendai virus
- From:
Chongqing Medicine
2017;46(1):90-93
- CountryChina
- Language:Chinese
-
Abstract:
Objective To comparatively study the features of two reprogramming systems of induced pluripotent stem cells (iPSCs)from human dental origin.Methods Two kinds of reprogramming system,i.e.STEMCCA lentivirus /feed layer and Sen-dai virus /matrigel were used to induce human stem cells from apical papilla(SCAP)into iPSCs,respectively.The induction efficien-cies,workload of generating iPSCs,aneuploidy karyotype ratio,complexities of eliminating exogenous transcription factors and spe-cific markers expression were compared between these two systems.Results The STEMCCA reprogramming system required to prepare the feeder cell MEF.The reprogramming efficiency was 0.1%.Transcription gene-free iPSCs cells were obtained by the Cre-loxp enzyme digestion technique at the later stage.Sendai virus reprogramming system was feeder-free and the preparation of matrigel was quite simple with unified standard.The reprogramming efficiency was 0.7%,which was much higher than that of STEMCCA system(P <0.05).The exogenous virus and transgenes could be gradually eliminated after several passages of natural subclone.Conclusion The Sendai virus/matrigle reprogramming system is much more applicable for the induction of iPSCs from dental origin than the STEMCCA system.
