Investigation of key miRNAs and their target genes in inflammatory bowel diseases and colitis-associated colorectal cancers using miRNA proifling and bioinformatic tools
10.19401/j.cnki.1007-3639.2016.11.006
- VernacularTitle:肠炎和肠炎相关结直肠癌miRNA表达检测及生物信息学分析
- Author:
Yuan YIN
;
Cheng WANG
;
Xin DAI
;
Zhaohui HUANG
- Keywords:
miRNA;
Ulcerative colitis;
Crohn’s disease;
Colitis-associated colorectal cancers;
Real-time lfuo-rescent quantitative polymerase chain reaction;
GO-analysis;
KEGG-analysis;
BIOCARTA-analysis
- From:
China Oncology
2016;26(11):916-921
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Inlfammatory bowel diseases (IBD) are a group of chronic intestinal diseases, including ulcerative colitis (UC) and Crohn’s disease (CD). This study identified differentially expressed miRNAs in UC, CD and colitis-associated colorectal cancers (CAC) to explore their potential as novel molecular biomarkers. Methods:Tissue samples were taken from 13 UC patients, 3 CD patients, 12 CAC patients, and 8 age-and gender-matched healthy controls. The miRNA expressions were detected by real-time fluorescent quantitative polymerase chain reaction (RTFQ-PCR) assay. Known targets of deregulated miRNAs were utilized using miRWalk 2.0 database, and subsequent bioinformatics analysis of these target genes was performed by DAVID software (GO-analysis, KEGG-analysis and BIOCARTA-analysis). Results:The data showed that miR-146a, miR-27a, miR-29a, miR-20a and miR-21 were upregulated in UC, CD and CAC tissues compared with normal control. Moreover, the target genes of these miRNAs were enriched in several key signal transduction pathways including cancer-related pathway and immu-nity-associated pathway. Conclusion:miR-146a, miR-27a, miR-29a, miR-20a and miR-21 may play important roles in the switching from IBD to CAC.
