Influence and significance of pristimerin therapy in patients with allergic asthma on Th17 and Treg
10.3969/j.issn.1000-484X.2017.01.011
- VernacularTitle:扁蒴藤素对支气管哮喘患儿Th17/Treg失衡的影响及意义
- Author:
Na CHEN
;
Jie HAN
;
Chunyan GAO
- Keywords:
Th17;
Treg;
Pristimerin;
Allergic asthma
- From:
Chinese Journal of Immunology
2017;33(1):58-61
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of pristimerin therapy on the functions of Th17 and Treg cells in patients with allergic asthma,and expore the function of immune factors in therapy on allergic asthma. Methods:Extracted mononuclear cells in pe-ripheral blood of patients with allergic asthma,and given pristimerin intervention in vitro. Then used fluorescence-activated cell sorting analysis to detect the contect of Th17 and Treg,enzyme-linked immunosorbent assay to detect cytokine production of IL-17 and TGF-β, and Real-time PCR to detect the contect of RORγT and Foxp3. Results: Compared with the control group, experimental group had reduced expression of CD4+IL-17+T cells ( Th17 cells) ,and increased expression of CD4+CD25+Foxp3+T cells ( Treg cells) ,which all had the statistical significance (P<0. 05);displayed decreased level of IL-17,and increased level of TGF-β,which all had the statistical significance (P<0. 05);had reduced expression of RORγT,and increased expression of Foxp3,which all had the statistical significance (P<0. 05). Conclusion: Pristimerin therapy can remit the unbalance of Th17/Treg in vitro,hinting this drug may regulate allergic asthma by influencing immunologic balance,which offers the theoretical support for pristimerin in clinical application.
