NDRG2 inhibits the proliferation of breast cancer cells via regulating β-catenin expression and nuclear translocation
10.19401/j.cnki.1007-3639.2016.12.004
- VernacularTitle:NDRG2通过抑制β-catenin表达和入核调控乳腺癌细胞增殖
- Author:
Xiaolei ZHOU
;
Chongyue ZHU
;
Shiguang ZHANG
;
Zhiyan ZHOU
;
Haichao LI
;
Wei ZOU
- Keywords:
Breast neoplasms;
N-myc downstream regulated gene 2;
β-catenin;
Cell proliferation
- From:China Oncology
2016;26(12):981-988
- CountryChina
- Language:Chinese
-
Abstract:
Background and purpose:Breast cancer is one of the most common malignant diseases in women and its malignant proliferation is the major cause of death. To investigate the effects of N-myc downstream regulated gene 2 (NDRG2) on proliferation of breast cancer cells by using two parallel cell lines (MCF-7 and LM-MCF-7) with different metastatic abilities.Methods:The expression level of NDRG2 in breast cancer cells was detected by Western blot. The effects of overexpressing (or down-regulating) NDRG2 on proliferation of breast cancer cells were investigat-ed by lfow cytometry. The expression and location of β-catenin were detected by Western blot and immunolfuorescence respectively. NDRG2 blocking the transcription activity of β-catenin was investigated via co-transfecting MCF-7 cells with NDRG2 siRNA and pCMV-Tcfδ (lacking the portion responsible for the protein binding to DNA).Results:The expression level of NDRG2 was negatively related to the proliferation ability of breast cancer cells. Over-expressing NDRG2 (or down-regulating) via transfecting LM-MCF-7 (or MCF-7) cells with pCMV-NDRG2 (or NDRG2 siRNA) could inhibit (or promote) cell proliferation. Interestingly, the results of Western blot, immunolfuorescence and lfow cytometry revealed that down-regulation of NDRG2 resulted from the down-regulation of β-catenin and blocking its nuclear translocation, which led to losing control of the proliferation of breast cancer cells.Conclusion:NDRG2 inhibit the proliferation of breast cancer cells via down-regulating the expression of β-catenin and blocking its nuclear translo-cation, which is signiifcant for exploring the molecular mechanism of proliferation of breast cancer cells.
