Preliminary Study on Serum Metabolites of Primary Biliary Cholangitis by Hydrogen Spectrum Nuclear Magnetic Resonance
10.3969/j.issn.1008-7125.2017.02.003
- VernacularTitle:原发性胆汁性胆管炎血清代谢物组的氢谱核磁共振初步研究
- Author:
Jing YANG
;
De DUAN
;
Huamei WU
;
Lihong YANG
;
Yan LI
;
Jinhui YANG
- Keywords:
Hydrogen Spectrum Nuclear Magnetic Resonance;
Liver Cirrhosis;
Biliary;
Diagnosis;
Glutamine;
Urocanic Acid
- From:Chinese Journal of Gastroenterology
2017;22(2):75-81
- CountryChina
- Language:Chinese
-
Abstract:
Hydrogen spectrum nuclear magnetic resonance (1 H-NMR ) is a commonly used method for metabolomics and has been applied in the study of liver cirrhosis and liver cancer,however,study on serum metabolites in patients with primary biliary cholangitis (PBC)is rare.Aims:To investigate the capability of 1 H-NMR for screening serum metabolites of PBC.Methods:Twenty PBC patients,20 HBV-related cirrhosis patients and 20 healthy controls were detected by 1 H-NMR.The 1 H-NMR spectra data were processed by principal component analysis (PCA)and orthogonal partial least squares-discriminant analysis (OPLS-DA)so as to create the diagnostic model.Based on the correlation coefficient P (corr),VIP value and non-paired t test of OPLS-DA model,the differential metabolites between normal group and the two cirrhosis groups were screened.Results:OPLS-DA model could effectively distinguish healthy controls and PBC patients,model interpretation ability and prediction ability were 81.9% and 44.8%,respectively (P=0.0293), glutamine,folic acid,urocanic acid,4-ethylbenzoic acid were differential metabolites.OPLS-DA model could also effectively distinguish healthy controls and HBV-related cirrhosis patients, urocanic acid, 1-methylhistamine, 1-methyladenine,glucose,L-acetylcarnitine were differential metabolites.OPLS-DA model could not effectively distinguish PBC patients and HBV-related cirrhosis patients.Conclusions:Serum glutamine and folic acid may be the potential biomarkers of PBC,which may be closely related to the immune damage mechanism and prognosis of PBC;1 H-NMR combined with OPLS-DA diagnostic model are expected to become a new method for studying liver cirrhosis.
