Effects of extracellular polysaccharides from Paecilomyces Lilacinuson on function of mouse bone marrow-derived dendritic cells
10.3969/j.issn.1000-484X.2017.02.010
- VernacularTitle:红树林淡紫拟青霉胞外多糖对小鼠DCs表型成熟的影响
- Author:
Haiyan HU
;
Huamin WANG
;
Yingzi LIN
;
Caihong CHANG
;
Wen YANG
;
Yongxia WANG
- Keywords:
Extracellular polysaccharides;
Paecilomyces Lilacinuson;
Dendritic cells;
Phenotype;
Maturation
- From:
Chinese Journal of Immunology
2017;33(2):212-216
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of the Paecilomyces Lilacinuson extracellular polysaccharides on the phenotypic and maturation of murine dendritic cells. Methods: Imature DCs were induced in vitro from the murine bone marrow cells in the presence of rmGM-CSF and rmIL-4, and then they were cultured with different dosage of the extracellular polysaccharides. The morphological characterization was analyzed under microscopy. The expressions of the DCs surface costimulating factors and phagocytic function to FITC-dextran were detected by flow cytometry. The level of IL-12 secreted by DCs was observed by ELISA. At the same time the influence of DCs on the proliferation of T cells was determined by MTT. Results:Treating with the polysaccharides for 48 h could up-regulate the expression of DCs surface molecules,such as CD11c,MHCⅡ,CD80 and CD86,and the 400 μg/ml was the optimal dose,comparing with the blank control group, the difference was significant (P<0. 01), contrast to LPS control group that was not different ( P<0. 05 ) . The uptaking FITC-dextran ability of the DCs treated with 300 μg/ml and 400 μg/ml polysaccharides was significant lower than the unstimulated DCs(P<0. 05). At the same time the extract at different concentration could distinctly enhance the proliferation of T cells by DCs too. Conclusion:The extracellular polysaccharides could stimulate the maturation of dendritic cells and induce the production of mature dendritic cells.
