Effects of miR-581 overexpression on proliferation of human colorectal cancer SW620 cells
10.3969/j.issn.1000-484X.2017.02.018
- VernacularTitle:miR-581对结直肠癌SW620细胞增殖能力的影响
- Author:
Longmei LI
;
Hongfei PAN
;
Hong ZHANG
;
Junmin LUO
;
Jihong FENG
- Keywords:
miR-581;
Colorectal cancer;
Proliferation
- From:
Chinese Journal of Immunology
2017;33(2):252-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of miR-581 overexpression on the proliferation of human colorectal cancer cell line SW620. Methods:The expression group,colorectal cancer SW620 cells were transfected with recombinant lentivirus vector ( LV-miR-581) and miR-581 mimics(miR-581),the negative control group were transfected with negative control lentiviral vector (LV-GFP) and negative control mimics (vector). The mRNA expression of miR-581 was identified by qRT-PCR. Proliferation of the cells were detected by CCK8 assary and colony forming assary. Results:The expression of miR-581 at mRNA significantly increased in LV-miR-581 group compared with control groups were detected by qRT-PCR ( P<0. 05 ) . Up-regulation of miR-581 markedly enhanced human colorectal cancer SW620 cells proliferation than those in the cells transfected with control vector ( P<0. 05 ) . Conclusion: Forced expression of miR-581 accelerates the proliferation of colorectal cancer SW620 cells.
