Role of protein tyrosine phosphatase SHP-2 in group I metabotropic glutamate receptors-induced allodynia
10.3969/j.issn.1001-1978.2017.02.006
- VernacularTitle:蛋白酪氨酸磷酸酶 SHP-2在Ⅰ型代谢型谷氨酸受体诱发痛觉超敏中的作用
- Author:
Man XUE
;
Yunhui YANG
;
Zhanwei SUO
;
Xian YANG
;
Xiaodong HU
- Keywords:
mGluR1 /5;
SHP-2;
phosphatase;
spinal cord dorsal horn;
DHPG;
NSC-87877
- From:
Chinese Pharmacological Bulletin
2017;33(2):171-174
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate whether the pain modi-fication by group I metabotropic glutamate receptors (mGluRs)required the involvement of Src homology-2 domain-containing phosphatase-2 (SHP-2 ).Methods Co-immunoprecipitation was performed to examine the possible interaction between SHP-2 and group I mGluRs in spinal cord dorsal horn of mice.By measur-ing the paw withdrawal thresholds,the effects of SHP-2 inhibitor NSC-87877 or its catalytically inactive SHP-2 (C459S ) mutant on allodynia induced by group I mGluRs agonist DHPG (50 nmol)were observed.Re-sults Anti-mGluR5 antibody was able to co-immuno-precipitate SHP-2 from spinal dorsal horn of mice, while no SHP-2 was precipitated by anti-mGluR1 anti-body.Inactivation of SHP-2 by NSC-87877 (6 nmol) or SHP-2 (C459S ) effectively attenuated allodynia caused by DHPG.Conclusion SHP-2 can physically interact with mGluR5.The activation of SHP-2 may be necessary for group I mGluRs to process the nocicep-tive information.
