Effects of bupivacaine on short-circuit currents in human alveolar epithelial cells
10.3969/j.issn.1001-1978.2017.02.011
- VernacularTitle:布比卡因对人肺上皮细胞短路电流影响的研究
- Author:
Yong CUI
;
Xuedong JIANG
;
Tong YU
;
Yan DING
;
Hongguang NIE
- Keywords:
bupivacaine;
short-circuit currents;
epi-thelial sodium channel;
protein phosphorylation;
pul-monary edema;
alveolar fluid clearance
- From:
Chinese Pharmacological Bulletin
2017;33(2):197-200
- CountryChina
- Language:Chinese
-
Abstract:
Aim Bupivacaine is a kind of long-acting amide local anesthetics.This paper aims to explore the effects of bupivacaine on the short-circuit currents in human alveolar epithelial monolayers and study the possible mechanisms.Methods Short-circuit currents were recorded by ussing-chamber setup.Amiloride-sensitive currents were defined as the difference be-tween the total current and the amiloride-resistant cur-rent.ERK1 /2 phosphorylation protein levels were ana-lyzed by Western blot at 0,1 5,30 and 60 min after administration of 1 00 μmol·L -1 bupivacaine.Results Bupivacaine could inhibit the short-circuit currents in H441 monolayers dose-dependently,which could be inhibited by amiloride.Western blot analysis showed that bupivacaine increased the level of ERK1 /2 phos-phorylation.Conclusion These data demonstrate that bupivacaine can reduce the alveolar ion transport by in-hibiting the amiloride-sensitive currents,possibly by the enhancement of ERK1 /2 phosphorylation. The effects of alveolar fluid clearance following application of bupivacaine should be considered clinically when the patient is complicated with lung injury.
