Fatty acid participates in up-regulation of diabetes on function and expression of CYP1A2
10.3969/j.issn.1001-1978.2017.02.020
- VernacularTitle:脂肪酸参与糖尿病状态下肝细胞上 CYP1A2功能与表达的上调
- Author:
Nan HU
;
Yan JIANG
;
Rui HAN
;
Qing QIAN
;
Sulan ZOU
- Keywords:
diabetes;
fatty acids;
HepG2;
Fa2N-4;
CYP1 A2 function;
CYP1 A2 mRNA expression
- From:
Chinese Pharmacological Bulletin
2017;33(2):249-254
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the mechanism of dia-betes changing the hepatic CYP1 A2 through in vitro cell culture study.Methods The function of CYP1 A2 in HepG2 and Fa2N-4 cells were evaluated by determi-ning the level of phenacetin metabolism,and the mR-NA expression of CYP1 A2 in cells was detected by real time PCR.HepG2 cells were co-cultured with serum of diabetic rats(type 1 and type 2)and normal rats,then the CYP1 A2 function in cells were evaluated.Then, the HepG2 and Fa2N-4 cells were co-cultured with a series of concentrations of saturated (including palmitic acid and stearic acid)and unsaturated fatty acids(in-cluding oleic acid and linoleic acid)for 48 h,and the function and expression of CYP1 A2 in the cells were compared.Results It was found that the activities of CYP1 A2 were higher in cells incubated with diabetic serum of both type.All high concentration of fatty acids could increase the function and expression of CYP1 A2 in both HepG2 and Fa2N-4 cells.Conclusion It is speculated that the abnormal level of fatty acids under diabetic state might be part of the reasons why diabetes change the hepatic CYP1 A2,which provides the basis for future study.
