In vitro release and gastrointestinal absorption of novel pyridostigmine bromide nanoemulsion
10.3969/j.issn.1001-1978.2017.02.024
- VernacularTitle:溴吡斯的明新型纳米乳体外释放和大鼠在体胃肠吸收
- Author:
Yuming YUAN
;
Xueliang CHEN
;
Jing CHEN
;
Na LI
;
Jingqing ZHANG
- Keywords:
pyridostigmine bromide;
novel pyridostig-mine bromide nanoemulsion;
drug release in vitro;
di-alysis method;
single-pass intestinal perfusion model;
gastrointestinal absorption
- From:
Chinese Pharmacological Bulletin
2017;33(2):276-279
- CountryChina
- Language:Chinese
-
Abstract:
Aim To prepare the novel pyridostigmine bromide nanoemusion(PPNE)and study its release in vitro, and to investigate the intestinal absorption. Methods Pyridostigmine bromide (PB)and PPNE were tested by HPLC in pH 1 .2 HCl,pH 6.8,pH 7.4,pH 7.8 PBS.Rat single pass intestinal perfusion method was employed to investigate the absorption mechanism of PB and PPNE.Results PB release rate was faster than PB in the four release media;the intes-tinal absorption rate constant(Ka )and apparent perme-ability coefficient(Papp)of PPNE were increased in the duodenum,jejunum,ileum and colon segments.PB and PPNE had significant difference in the duodenum, jejunum,ileum and colon segments by t test (P <0.05).Conclusions PPNE can improve the bioavail-ability of drugs,increase the drugs permeability,sig-nificantly improve the absorption of the drugs in the in-testinal segments. PPNE has obviously sustained effects.
