Study on Expressions of Hydrogen Sulfide-producing Enzymes in Esophagus of Patients with Achalasia
10.3969/j.issn.1008-7125.2017.02.005
- VernacularTitle:贲门失弛缓症患者食管硫化氢合成酶表达的研究
- Author:
Lili ZHANG
;
Wei ZHAO
;
Hong JIN
;
Bangmao WANG
- Keywords:
Esophageal Achalasia;
Hydrogen Sulfide;
Hydrogen Sulfide-Producing Enzymes;
Immunohistochemistry
- From:
Chinese Journal of Gastroenterology
2017;22(2):87-90
- CountryChina
- Language:Chinese
-
Abstract:
Achalasia is a primary esophageal motility disorder.It has been widely accepted that the occurrence of achalasia is associated with reduction of inhibitory neurotransmitters.Hydrogen sulfide,which is known as a gastrointestinal inhibitory neurotransmitter,is produced endogenously from L-cysteine mainly via cystathionine beta-synthetase (CBS)and cystathionine gamma-lyase (CSE).Aims:To explore the relationship between hydrogen sulfide and achalasia by comparing the expressions of CBS and CSE in esophagus of patients with achalasia and the control subjects.Methods:Twenty-five achalasia patients undergoing peroral endoscopic myotomy from Jan.2013 to Jun.2015 at the Tianjin Medical University General Hospital were enrolled.Circular muscle samples in lower esophageal sphincter (LES)were obtained for detection of CBS and CSE immunohistochemically,and normal LES circular muscle samples from 10 patients with esophageal squamous carcinoma were served as controls.Reductions of CBS and CSE were evaluated semi-quantitatively.Results:Immunoreactivities for CBS and CSE were observed mainly in myenteric nerve plexus of LES.Compared with control subjects,expressions of CBS and CSE in LES of achalasia patients were significantly reduced (P all <0.05).More than 60%of the achalasia patients revealed severe reduction to complete loss of CBS and CSE immunoreactivities,whereas most of the control subjects were strongly positive for these two enzymes.Conclusions:Hydrogen sulfide-producing enzymes are significantly reduced and even absent in LES of patients with achalasia,which indicates that there exists a correlation between reduced hydrogen sulfide synthesis and achalasia.
