The significance of cytometric beads array for the detection of Th1/Th2/Th17 cytokine in the serum of patients with pulmonary tuberculosis
10.3969/j.issn.1673-4130.2017.02.001
- VernacularTitle:流式微球分析法检测血清中 Th1／Th2／Th17型细胞因子的表达在肺结核病患者中的临床意义
- Author:
Xiaoxiao ZHANG
;
Huifang ZHOU
;
Zhaoxia ZHANG
;
Qiong ZHANG
;
Cunren MENG
- Keywords:
cytometric beads array;
tuberculosis;
Th1/Th2/Th17;
cytokines
- From:
International Journal of Laboratory Medicine
2017;38(2):145-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the significance of cytometric beads array(CBA)in the detection of Th1/Th2/Th17 cytokine in patients with pulmonary tuberculosis.Methods The levels of 7 cytokines (IL-2,IL-4,IL-6,IL-10,TNF,IFN-γ and IL-17A) were detected by CBA according to the instruction of BD CBA Human Th1/Th2/Th17 Cytokine Kit(BD Biosciences,USA)in the serum of 98 patients with tuberculosis(38 sputum-smear positive and 60 sputum-smear negative)and 79 healthy individuals.Then analyze the relationship and differences among these groups.Results Except IL-17A,the level of IL-2,IL-4,IL-6,IL-10,TNF,IFN-γwas higher in the patients group than control group;As to IL-2,IL-4,IL-10 and IFN-γ,the sputum-smear positive group had a higher level than control group,but the sputum-smear positive group had no difference with sputum-smear negative group.Com-pared with sputum-smear negative group,the sputum-smear positive group have a higher level of IL-6 and TNF.Conclusion IL-6 and TNF can be used to monitor the prognosis of tuberculosis.Meanwhile,CBA is sensitive,could detect several cytokines once with less sample consumption and time,thus could be applied for the monitor of tuberculosis progress.
