Effect of lipidemia on time-resolved fluoroimmunoassay for determination of unconjugated estriol
10.3969/j.issn.1673-4130.2017.02.016
- VernacularTitle:脂血标本对时间分辨荧光免疫分析法测定游离雌三醇水平的影响
- Author:
Guoqing WANG
;
Jun ZHAO
;
Yuqiu ZHOU
- Keywords:
lpidemia;
time-resolved fluoroimmunoassay;
unconjugated estriol
- From:
International Journal of Laboratory Medicine
2017;38(2):189-190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different degree lipidemia on time-resolved fluoroimmunoassay(TRFIA)for determination of unconjugated estriol(uE3).Methods Mixed serum was prepared by collecting different levels of lipidemia samples which were normal and chylous appearance from male and by mixing with definite value serum of uE3.The levels of uE3 in the sam-ples were measured by TRFIA and the effect of lipidemia on TRFIA for determination of uE3 was evaluated.Results For the ap-pearance of chylous specimens,mild lipidemia increased uE3,mid-or hiper-lipidemia samples reduced uE3 and the effect of both was considerable.Conclusion The chylous lipidemia has variant degree of influence to TRFIA for determination of uE3,then the results effect accuracy of Down′s screening.