Research on nucleotide sequence of a newly emerged pandemic norovirus GⅡ.4 genotype
10.3969/j.issn.1673-4130.2017.02.032
- VernacularTitle:诺如病毒 GⅡ．4亚型新变异株全基因组序列的研究
- Author:
Mingli ZHOU
;
Ailing CAI
;
Xuefeng WANG
- Keywords:
Norovirus;
variant strain of Sydney 2012 GⅡ.4;
complete genome sequences
- From:
International Journal of Laboratory Medicine
2017;38(2):231-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective Analysis of the complete genome sequence about the newly emerged pandemic norovirus GⅡ.4 genotype, to understand its variation characteristics.Methods 264 patients were collected with diarrhea.The RNA was extracted from 264 fe-cal specimens and cDNA was synthetized.The positive samples were amplified by PCR,the amplified fragments were sequenced. The complete genome sequences of the norovirus was sequenced and analyzed.Results A new norovirus variant strain of Jingzhou GⅡ.4,a pleiston of Sydney GⅡ.4 was isolated.A large variation was found in the new variant subtype,which was mutated inclu-ding in the hypervariable P2 domain of the major capsid protein VP1.Conclusion The result demonstrates the variant strain of Sydney GⅡ.4 was spread to China.VP1 of norovirus GⅡ.4 is evolving rapidly.The spread and evolution situation of the norovirus GⅡ.4 need to be closely monitored in China for the development of effective vaccines and therapeutic monoclonal antibodies.
