Simultaneous determination of liquiritin, glycyrrhizic acid and glycyrrhetinic acid in Xianlong-Jiedu Yin by HPCE
10.3760/cma.j.issn.1673-4246.2017.02.013
- VernacularTitle:高效毛细管电泳法测定仙龙解毒饮中甘草苷、甘草酸及甘草次酸含量
- Author:
Juanjuan ZHOU
;
Jun QI
;
Yadong QIN
- Keywords:
Capillary electrochromatography;
Liquiritin;
Glycyrrhizic acid;
Glycyrrhetinic acid;
Xianlong-Jiedu Yin
- From:
International Journal of Traditional Chinese Medicine
2017;39(2):144-147
- CountryChina
- Language:Chinese
-
Abstract:
Objective To simultaneously determine the quantify liquiritin, glycyrrhetinic acid and glycyrrhizic acid inXianlong-Jiedu Yin by high performance capillary electrophoresis (HPCE).Methods HPCE method was performed on a fused silica capillary coates 75μm×85 cm (effective column length 78.5 cm) using 50 mmol/L borax solution-acetonitrile (95:5), with adjusted pH=8.50 as running buffer, lytical 22 kV, and electrokinetic injection 10 s, detected wave length of 254 nm, and temprature maintaining 25℃.Results The liquiritin, glycyrrhetinic acid and glycyrrhizic acid were completely separated within 30 minutes, showing good linear relationship in the range of 12-120, 15-150, 8-160μg/ml (r>0.999 3). The average recoveries were 100.70%, 98.51%, 98.40% withRSD 2.49%, 2.79%, 0.40%, respectively.Conclusions The HPCE was suitable for the determination of liquiritin, glycyrrhetinic acid and glycyrrhizic acid inXianLong-JieDu Yin, which can be used for the quality control.
