In Silico Cloning and Bioinformatics Analysis of AP2/ERF Family Gene from Brassica Oleracea
- VernacularTitle:甘蓝AP2/ERF转录因子的克隆和生物信息学分析
- Author:
Hui GUO
;
Siyang JIN
;
Han LIU
;
Zhenyue WANG
- Keywords:
In silico cloning;
Brassica oleracea;
AP2/ERF;
Bioinformatics
- From:
China Pharmacist
2017;20(1):6-10
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To obtain AP2/ERF genes from Brassica oleracea by using in silico cloning. Methods: AP2/ERF genes were cloned by retnieving the EST database and using the bioinformatics software with the Arabidopsis thaliana AP2/ERF as a querying probe. Results:Two AP2/ERF family transcriptional regulators (BoAP2/ERF1 and BoAP2/ERF2) were isolated from Brassica olera-cea by the in silico cloning method. Some characters of AP2/ERF protein were analyzed and predicted by the tools of bioinformatics in the following aspects including the composition of amino acid sequence, hydrophilicity and hydrophobicity, subcellular localization, secondary and tertiary structure of protein and function. Conclusion:Bioinformatical analysis shows BoAP2/ERF1 and BoAP2/ERF2 gene encode 40. 85kDa and 39. 44kDa protein with 371 and 352 amino acids. The domain is predicted to locate on nucleus. Sequence analysis indicates the protein may be involved in signaling transducer and stressing response roles in plantbiotic stresses.
