Optimization of Extraction Process of Berberine Hydrochloride from Sanhuang Diyu Oil by Box-Benhnken Response Surface Methodology
- VernacularTitle:Box-Benhnken响应面法优化三黄地榆油中盐酸小檗碱的提取工艺
- Author:
Qiuting GUO
;
Xiaofei ZHANG
- Keywords:
Sanhuang Diyu oil;
Berberine hydrochloride;
Box-Behnken response surface methodology;
Extraction Process
- From:
China Pharmacist
2017;20(1):56-59
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To optimize the extraction process of berberine hydrochloride from Sanhuang Diyu oil by Box-Benhnken re-sponse surface methodology. Methods:The extraction process was optimized with the size of each medicine ( X1 ) , the liquid-solid ra-tio ( X2 ) and the extraction time ( X3 ) as the independent variables and berberine hydrochloride extraction amount ( Y) as the depend-ent variable, and the effects of variables and their interactions on the extraction efficiency were studied. Results:The experiment results indicated that the optimal extraction conditions were as follows:the size of each medicine was 60 meshes, the liquid-solid ratio was 18, and the extraction time was 1. 5 h. Under the above conditions, 3 batches of berberine hydrochloride from Sanhuang Diyu oil were ex-tracted. The average extraction rate of berberine hydrochloride was (16.6 ±0.6) mg·g-1(n =3). Conclusion: The extraction process of Sanhuang Diyu oil optimized by Box-Behnken response surface methodology is simple, stable and predictable.
