Preparation of VEGFRⅡ-mediated Targeted Microbubble Image Agent and its Cancer Targeting Ability in vitro
- VernacularTitle:VEGFR Ⅱ介导靶向微泡显像剂的制备及体外肿瘤靶向性实验研究
- Author:
Ying CHEN
- Keywords:
Vascular endothelial growth factor receptorⅡ;
Targeted microbubble image agent;
Membrane dispersion-ultrasonic method;
Immunofluorescent
- From:
China Pharmacist
2017;20(1):73-76
- CountryChina
- Language:Chinese
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Abstract:
Objective:To prepare vascular endothelial growth factor receptorⅡ ( VEGFRⅡ)-mediated targeted sulphur hexafluo-ride microbubble image agent and evaluate its targeting ability in human breast cancer MCF-7 in vitro. Methods:The sulphur hexafluo-ride microbubble image agent was prepared by a membrane dispersion-ultrasonic method and optimized by Box-Behnken design using design-expert 8. 0. 6 software. VEGFRⅡ-mediated targeted microbubble image agent was prepared by an absorption method. VEGFRⅡ-mediated targeted microbubble image agent was characterized by the morphology, particle size distribution and zeta potential. The targeting ability of the image agent in human breast cancer MCF-7 in vitro was detected by an immunofluorescent assay. Results:The particle size, polydispersion index and zeta potential of VEGFRⅡ-mediated targeted microbubble image agent was (3. 81 ± 0. 32) μm, 0. 261 ± 0. 037 and ( -25. 7 ± 2. 8) mV, respectively. The microbubbles were small and spherical with smooth surface as seen under a transmission electron microscope. VEGFRⅡ-mediated targeted microbubble image agent could combine with human breast cancer MCF-7 specifically in vitro. Conclusion: The absorption preparation technology of the targeted microbubble image agent is feasible, and the microbubble image agent has strong and especial targeting function in vitro.
