Qualitative Identification and Content Determination of Aurantio-obtusin and Chrysophanol in Zeju Jiangzhi Tablets
- VernacularTitle:泽菊降脂片中橙黄决明素和大黄酚的定性鉴别和含量测定
- Author:
Dong LIU
;
Xiaohong SONG
;
Lijun WANG
;
Changming TANG
;
Mingchun LI
;
Yanqin CHENG
- Keywords:
Zeju Jiangzhi tablets;
Aurantio-obtusin;
Chrysophanol;
TLC;
HPLC
- From:
China Pharmacist
2017;20(1):163-165
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the methods for the qualitative identification and content determination of aurantio-obtusin and chrysophanol in Zeju Jiangzhi tablets. Methods:A TLC method was adopted for the qualitative identification, and an HPLC method was used for the content determination. The determination was performed on a Wondasil C18 (250 mm × 4. 6 mm, 5 μm ) column with the mobile phase of acetonitrile -0. 1% phosphonic acid with gradient elution at the flow rate of 1. 0 ml?min-1 , the detection wave-length was 286 nm, the column temperature was 30℃ and the injection volume was 10μl. Results:The TLC spots of aurantio-obtusin and chrysophanol were clear and well-separated without any negative interference. The HPLC experiment results showed the good line-arity within the range of 1. 03-25. 72μg?ml-1(r=0. 999 9) for aurantio-obtusin, and 0. 48-11. 92μg?ml-1(r=0. 999 9) for chry-sophanol. The average recovery was 99. 21% and 98. 85%, and RSD was 0. 70% and 0. 73%, respectively (n=9). Conclusion:The method is simple, accurate and repeatable, which can be used for the qualitative identification and content determination of auran-tio-obtusin and chrysophanol in Zeju Jiangzhi tablets.
