Development of a Noble Dosimetry Using Metaphase Analysis and Micronuclei Assay of Bone Marrow Cells in Mice.
- Author:
Jung Jun MIN
;
Hee Seung BOM
;
Young Ho KIM
;
Hyun Joong YOON
;
Ji Yeul KIM
- Publication Type:Original Article
- Keywords:
Bone marrow;
Chromosomal aberration;
Micronucleus;
Metaphase analysis;
Micronuclei assay;
Biological dosimetry
- MeSH:
Animals;
Bone Marrow Cells*;
Bone Marrow*;
Chromosome Aberrations;
Metaphase*;
Mice*;
Micronucleus Tests
- From:Korean Journal of Nuclear Medicine
2000;34(1):74-81
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The purpose of this study was to develop in vivo dosimetries using both chromosomal aberrations and micronuclei in mice to assess biological effects of radiations. MATERIALS AND METHODS: Five each mice were irradiated with 0, 1, 2, 3, 4, 5, 10 Gy of Cs-137 gamma-rays. We scored numbers of chromosomal aberrations in metaphase spreads and numbers of micronuclei in bone marrow smears under light microscope, and obtained the dose-response relationships. We also examined the relationship between the two dose-response curves. RESULTS: The frequency of both chromosomal aberrations and micronuclei increased with dose, in a linear-quadratic manner. The delta, beta, and alpha coefficients were 0.0176, 0.0324, and 0.0567 for metaphase analysis (r=1.0, p<0.001) and 0.0019, 0.0073, and 0.0506 for micronuclei assay (r=1.0, p<0.001). The frequency of chromosomal aberrations and micronuclei in diffirent radiation doses was significantly correlated (r=0.99, p<0.01). CONCLUSION: In vivo dosimetry using either metaphase analysis or micronucleus assay was feasible in mice. These methods could be useful to evaluate biological effects of radiation.
