Analysis of Diosgenin Content in Different Processed Products of Polygonatum from Shaanxi
- VernacularTitle:陕西产黄精不同炮制品中薯蓣皂苷元含量分析
- Author:
Tingting SUN
;
Hong ZHANG
;
Ye LI
;
Chunliu WANG
;
Zhenzhen HAO
;
Maixia MENG
;
Ganghu REN
- Keywords:
Polygonatum;
Process;
Diosgenin;
HPLC;
Content determination
- From:
China Pharmacist
2017;20(1):158-160
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the effects of different processes on diosgenin content in polygonatum from Shaanxi. Methods:The content of diosgenin was determined by HPLC on a column of Woburn C18 (250 mm × 4. 6 mm, 5 μm) with the mobile phase of acetonitrile-water(90 ∶10)at the flow rate of 1.0 ml·min-1, the detection wavelength was 203 nm, the column temperature was 30℃, and the injection volume was 30 μl. Results:The linear range of diosgenin was 0. 892-5. 352 μg(r=0. 999 9), and the aver-age recovery was 99. 5% (RSD=2. 53%). The content of diosgenin was significantly various among the samples of polygonatum with different processes from Shaanxi (P<0. 05), and that in the raw slices was the highest followed by the steam samples, while that in al-cohol evaporate slices was the lowest. Conclusion:The results of the research can provide reference for the quality control and process-ing methods of polygonatum from Shaanxi.
