Can dida colonizait on associated with the humoral immune anamnestci respon se oft he dominant antigen in the early stage of invasive Candidosis
10.16571/j.cnki.1008-8199.2017.01.005
- VernacularTitle:念珠菌定植引发侵袭性感染早期优势抗原的体液免疫回忆应答
- Author:
Xiujuan SHANG
;
Xiao CHEN
;
Fangqiu LI
;
Yuan HU
;
Lining SHI
;
Qian LIU
- Keywords:
Candida infection;
Humoral immunity;
Memory B cells;
ELISPOT
- From:
Journal of Medical Postgraduates
2017;30(1):21-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective Rapid elevation of the IgG antibody against Candida Enolase ( Eno ) has been observed in patients with invasive candidosis in an early stage .The present study was to confirm the association of Candida colonization with humoral im-mune anamnestic response of the dominant antigen . Methods Twenty-four mice were randomized into group 1 treated by oral Candi-da colonization plus intraperitoneal infection ( immunocompetent , n=8) , 2 treated with immunosuppressant in addition to the treatment of group 1 ( immunocompromised , n=8) , 3 treated by oral Candida colonization only ( immunocompetent , n=4) and 4 treated by in-traperitoneal injection only( immunocompetent, n=4).The number of Eno-specific memory B-cells in the spleen and the levels of IgG , IgM and IgA antibodies were determined in the peripheral blood of the immunocompetent and immunocompromised invasive candidiasis mice . Results At 7 days after invasive infection , there were significantly more Eno-specific memory B-cells in the mice of groups1 ( 47.25 ± 13.81) and 2 (43.14±15.95) than in groups 3 (8.00±3.74) and 4(8.50±2.38) (P<0.01), with no statistically significant differences between either groups 1 and 2 or groups 3 and 4 (P >0.05).Eno-IgG antibodies were detected in the serum of the mice of the first two groups in the early stage of invasive infection and positively corre -lated with antigen-specific memory B-cells (r=0.737,P <00.1 ). Conclusion Rapid elevation of the Eno-IgG antibody level in the early stage of invasive infection after Candida colonization may be attributed to the rapid proliferation of humoral immune memory cells.
