Determination of Erythromycin in Erythromycin Enteric Capsules by UV
- VernacularTitle:紫外分光光度法测定红霉素肠溶胶囊中红霉素的含量
- Author:
Xiaoshu NIU
;
Huafeng YUAN
- Keywords:
Erythromycin;
Enteric capsules;
Sodium hydroxide;
UV spectrophotometry;
Determination
- From:
China Pharmacist
2017;20(1):169-171
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a method for the determination of erythromycin in erythromycin enteric capsules. Methods:UV spectrophotometry was performed. Sodium hydroxide reacted with erythromycin to obtain a unsaturated ketone having maximum absor-bance at 235 nm. The content of erythromycin in erythromycin enteric capsules was determined by the UV absorbance of the unsaturat-ed ketone. Results: The linear range of erythromycin was 51. 18-307. 08 μg · ml-1 ( r =0. 999 9 ) and the average recovery was 99. 9%(RSD=1. 2%,n=6). Conclusion:The method is simple,accurate,sensitive and reproducible, which can be used for the de-termination of erythromycin in erythromycin enteric capsules.
