Quality Standard for Huayu Pills
- VernacularTitle:化瘀丸质量标准的建立
- Author:
Ting LI
;
Liu YANG
;
Yisheng ZHANG
;
Wei WANG
;
Xiaojing SUN
;
Li LI
;
Xiaoxia XIE
- Keywords:
Huayu pills;
Quality standard;
TanshinoneⅡA;
Chrysophanol;
Physcion
- From:
China Pharmacist
2017;20(1):179-181
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the quality standard for Huayu pills. Methods:TLC was adopted to identify salvia miltiorrhi-za, rhubarb charcoal and panax notoginseng; the contents of tanshinoneⅡA , chrysophanol and physcion in Huayu pills were deter-mined by RP-HPLC with Wondasil C18 (250 mm × 4. 6 mm,5μm) as the analytical column, the mobile phase was composed of metha-nol-0. 1% phosphoric acid solution (85 ∶15), the flow rate was 1. 0 ml·min-1,the detection wavelength was 254 nm, the column temperature was 35℃, and injection volume was 10 μl. Results:The TLC spots were clear and well-separated without any negative in-terference; the calibration curve of tanshinoneⅡA , chrysophanol and physcion was linear over the range of 0. 212-2. 12 μg ( r =0. 999 9),0. 159-1. 59 μg(r=0. 999 9) and 0. 072 6-0. 726 μg(r=0. 999 9), the average recovery was 99. 55 %, 99. 56% and 100. 60%, and RSD was 1. 57%,2. 26% and 2. 28%(n=6), respectively. Conclusion: The method is fast and accurate with good specificity, and can be used for the quality control of Huayu pills.
