Qualitative and Quantitative Determination Methods for Vinegar Frankincense in Liuwei Jingkang Capsules
- VernacularTitle:六味颈康胶囊中醋乳香定性定量方法的建立
- Author:
Guiqiang FAN
;
Hongmei GENG
;
Na MA
;
Hongxia PANG
- Keywords:
Liuwei Jingkang capsules;
Vinegar frankincense;
TLC;
Acetyl-11-keto-β-boswellic acid;
HPLC
- From:
China Pharmacist
2017;20(1):192-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a quality standard for vinegar frankincense in Liuwei Jingkang capsules. Methods: TLC was used for the identification of vinegar frankincense. HPLC was used for the content determination of acetyl-11-keto-β-boswellic acid (AKBA), which was the main active component in vinegar frankincense. A SHIMADZU Shim-pack VP-ODS(250 mm × 4. 6 mm,5μm) column was used. The mobile phase consisted of acetonitrile and water containing 0. 01 mol·L-1 hydrochloric acid (78 ∶22) at a flow rate of 1. 5 ml·min-1 . The column temperature was 30℃. The detection wavelength was 252 nm, and the injection volume was 10 μl. Results:The TLC method could identify the characteristic fluorescence of vinegar frankincense was without interference from the blank. There was a good linear relationship of AKBA within the concentration range of 0. 036 5-0. 730 8 mg·ml-1(r=0. 999 7). The average recovery was 98. 24% (RSD=0. 83%, n=9). Conclusion:The established method is accurate, highly sensitive and well re-producible, which can be used for the quality control of Liuwei Jingkang capsules.
