Effect of ketamine on mitochondrial function of rat neurons subjected to anoxia
10.3760∕cma.j.issn.0254-1416.2016.11.031
- VernacularTitle:氯胺酮对大鼠神经元缺氧时线粒体功能的影响
- Author:
Mingfeng LIAO
;
Kun CHEN
;
Zhifa ZHANG
;
Lu LI
;
Ailin LUO
;
Yuke TIAN
;
Xueren WANG
- Keywords:
Ketamine;
Hypoxia,brain;
Neurons;
Mitochondria
- From:
Chinese Journal of Anesthesiology
2016;36(11):1400-1402
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of ketamine on the mitochondrial function of rat neurons subjected to anoxia. Methods Primarily cultured rat hippocampal neurons were seeded in culture dishes (35 mm in diameter) at the density of 5×105-1×106 cells∕ml, and divided into 3 groups (n=11 each) using a random number table: control group, anoxia group and ketamine group. The neurons were exposed to 90% N2 plus 10% CO2 50 ml∕min for 5 min in anoxia group. In ketamine group, ketamine was added to the culture medium with the final concentration of 20 μmol∕L at 1 h before anoxia, and then the neurons were exposed to 90% N2 plus 10% CO2 50 ml∕min for 5 min. After the end of treatment in each group, the dead neurons were detected using trypan blue staining, the ATP content was determined by ATP bioluminescence assay, and mitochondrial membrane potential was measured by rhodamine 123 staining. Results Compared with control group, the mortality rate of hippocampal neurons was significantly in?creased, and the ATP content and mitochondrial membrane potential were significantly decreased in anoxia group and ketamine group ( P<0.05) . Compared with anoxia group, the mortality rate of hippocampal neu?rons was significantly decreased, and the ATP content and mitochondrial membrane potential were signifi?cantly increased in ketamine group (P<0.05). Conclusion The mechanism by which ketamine amelio?rates anoxia?induced damage to rat neurons is related to improved mitochondrial function.
