Study on Chemical Compounds and in vitro Antibacterial and Antioxidant Activities of Monoraphidium dy-bowskii
10.6039/j.issn.1001-0408.2017.04.09
- VernacularTitle:单针藻的化学成分及其体外抗菌、抗氧化活性研究
- Author:
Zhenyu ZHAO
;
Ning LUO
;
Chen CHEN
;
Ang LI
;
Shasha MA
;
Jiguang LIU
;
Meng WANG
;
Pinghuai LIU
- Keywords:
Monoraphidium dybowskii;
Ethyl acetate;
Extract;
Chemical components;
Identification;
Antibacterial;
Antioxi-dant
- From:
China Pharmacy
2017;28(4):465-468
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study chemical compounds of Monoraphidium dybowskii,and to investigate the in vitro antibacte-rial and antioxidant activities of isolated compounds. METHODS:The ethanol extract of M. dybowskii were extracted with aether petrolei,ethyl acetate and n-butyl alcohol. The ethyl acetate extract was separated from M. dybowskii and chemical components were analyzed by sillica gel column chromatogram,HPLC and GC-MS. Their structures were identified according to physicochemi-cal properties and NMR. MIC of 4 isolated compounds to Pseudomonas aeruginosa,Candida albicans,Bacillus subtilis and Esche-richia coli were determined by resazurin disc test. Free radical scavenging rate(concluated by IC50)and reducing capacity were mea-sured by 1,1-diphenyl-2-picryl-diazanyl (DPPH) and ferric reducing antioxidant power (FRAP) assay. RESULTS:Compounds 1-6 were obtained from E4 and E5 segments of ethyl acetate extract of M. dybowskii,and their structures were identified as stigmas-terol,diisonoyladipate,indole-3-carboxylic acid,(+)-epiloliolide,(-)-loliolide,5-hydroxy-3,4-dimethy-5-pentyl-2(5H)-furanone. MIC of compounds 3-6 were 10-500 μg/mL,and IC50 ranged 22.02-71.01 μg/mL;FRAP ranged (62.04 ± 5.36)-(281.22 ± 8.3) μmol/L. CONCLUSIONS:M. dybowskii contains multiple lipid and alkanoic acid,and possesses certain in vitro antibacterial and antioxidant activities.
