Research on carrying situation of Clostridium difficile toxin gene cdtA/B in stool of patients with ulcerative colitis
10.3969/j.issn.1673-4130.2017.04.002
- VernacularTitle:溃疡性结肠炎患者粪便中艰难梭菌毒素基因A/B携带情况的研究
- Author:
Min NIU
;
Shumin LIU
;
Yan DU
- Keywords:
ulcerative colitis;
Clostridium difficile;
toxin gene
- From:
International Journal of Laboratory Medicine
2017;38(4):436-438
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detection the carrying situation of Clostridium difficile toxin gene A/B in stool specimens of the pa-tients with ulcerative colitis (UC) and persons undergoing physical examination and to research the relationship between Clostridium difficile toxin gene carrying and UC.Methods The stool specimens were collected from 53 cases of UC(32 cases of active stage and 21 cases of resting stage) and 45 persons undergoing physical examination.Total DNA was extracted from stool specimens.The Clostridium difficile toxin gene cdtA and cdtB were detected by real-time PCR ,then the PCR products were amplified and performed the agarose gel electrophoresis and gene sequencing for conducting the amplification products verification.Results 7 cases of cdtA and 4 cases of cdtB were checked out in the UC group ,in which 5 cases of cdtA and 2 cases of cdtB were in the UC active stage group ,2 cases of cdtA and 2 cases of cdtB were in the UC resting stage group.In the healthy control group ,5 cases of cdtA and 2 cases of cdtB were checked out.The detection rate of cdtA and cdtB had no statistically significant difference between the UC group and healthy control group(P>0.05).There was no statistically significant difference between UC active group and inactive group (P>0.05).Conclusion There is no appearant correlation between the carrying situation of cdtA and cdtB in stool with UC onset and UC stage.
