Study on in vitro drug susceptibility test of biomembrane-producing Klebsiella pneumoniae
10.3969/j.issn.1673-4130.2017.04.023
- VernacularTitle:产生物膜的肺炎克雷伯菌的体外药敏试验探讨
- Author:
Zongsheng SHI
;
Shicong XUE
- Keywords:
bacterial biofilm;
Klebsiella pneumoniae;
K-B disk diffusion method;
drug sensitivity test
- From:
International Journal of Laboratory Medicine
2017;38(4):490-492
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the difference of in vitro drug susceptibility test of biomembrane-producing Klebsiella pneumoni-ae to provide accurate drug reference for clinical treatment.Methods The drug susceptibility test was carried out in clinically isola-ted and screened16 strains of biomembrane-producing Klebsiella pneumoniae by K-B paper diffusion method ,and the results at 24 , 48 ,72 h were recorded.Then the strains were reproduced for several generations until not producing biofilm.Then the above opera-tions were taken again.Finally ,the results of twice drug susceptibility tests were performed the contrastive analysis.Results The mm number of inhibition zone of biomembrane-producing Klebsiella pneumoniae at 24 h had statistical difference compared with that at 48 ,72 h(P<0.05).Conclusion When the in vitro drug susceptibility test of biomembrane-producing Klebsiella pneumoniae is conducted by K-B paper diffusion method ,it is suitable to report the results after 48 h ,this has a certain reference value for pre-venting this bacterium.
