The expression and clinical significance of tumor suppressor phosphatase -tension protein gene in differentia-ted thyroid carcinoma
10.3760/cma.j.issn.1008-6706.2017.04.014
- VernacularTitle:同源磷酸酶-张力蛋白基因在分化型甲状腺癌组织中的表达及意义
- Author:
Zhen WANG
;
Jingyu WANG
;
Zhiqin GUO
;
Lihui LU
- Keywords:
Thyroid neoplasms;
Genes,suppressor;
Mutation
- From:
Chinese Journal of Primary Medicine and Pharmacy
2017;24(4):536-539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the expression and clinical significance of tumor suppressor phosphatase -tension protein gene(PTEN)in differentiated thyroid carcinoma and the relationship with the V600E gene site muta-tion of murine sarcoma viral oncogene homolog B1(BRAF).Methods The expression of PTEN was assayed by im-munohistochemical staining in differentiated thyroid carcinoma and adjacent tissues.The differences were compared between clinical pathological features.The BRAF V600E gene mutation was detected by fluorescence quantitative pol-ymerase chain reaction(PCR)and to investigate the correlation between the expression of PTEN and BRAF V600E mutation.Results The negative rate of PTEN was 78.75%(63 /80)in differentiated thyroid carcinoma,13.75%(11 /80)in adjacent tissues(χ2 =27.236,P =0.000).There were significant differences in the expression of PTEN between I -II and III -IV in TNMstage,grade I and II,and lymph node metastasis(χ2 =10.395,6.948,9.263,P =0.000,0.006,0.000).The differences were not significant in the expression of PTEN between different gender,≤45 years and >45 years different pathological types and tumor diameter <2cm and ≥2cm(χ2 =1.113,0.941, 2.301,1.567,P =0.185,0.213,0.087,0.181).There was correlation between the expression of PTEN and BRAF V600E mutations(r =0.301,P =0.004).Conclusion The expression of PTEN is involved in the occurrence and development of differentiated thyroid cancer and is associated with BRAF V600E.The PTEN has the predictive value of prognosis in tumors.
