Effect of serum of patients with obstructive jaundice on myogenic differentiation of human pulmona-ry microvascular endothelial cells
10.3760∕cma.j.issn.0254-1416.2016.09.027
- VernacularTitle:梗阻性黄疸患者血清对人肺微血管内皮细胞肌样分化的影响
- Author:
Qiwei CHEN
;
Yong YANG
;
Bing CHEN
;
Yang CHEN
;
Sheng LI
;
Guilan WANG
;
Yulong WU
;
Chonghui CHEN
;
Baoli ZU
;
Bin YI
;
Kaizhi LU
;
Lin LIAO
- Keywords:
Jaundice,obstructive;
Serum;
Lung;
Endothelium,vascular;
Cell differentia-tion
- From:
Chinese Journal of Anesthesiology
2016;36(9):1146-1149
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of the serum of patients with obstructive jaundice on myogenic differentiation of human pulmonary microvascular endothelial cells (PMVECs). Methods Hu?man PMVECs were isolated and then subcultured. The cultured PMVECs were incubated with the serum of patients with obstructive jaundice or with the serum of healthy volunteers. At 24, 48 and 72 h of incubation (T1?3), the inverted microscope was used to observe the morphology of primary PMVECs. The expression of muscular proteins ( alpha?smooth muscle actin [α?SMA ] , smooth muscle?mysion heavy chain [ SM?MHC] , capolnin) in PMVECs was detected using Western blot analysis. Results The expression of cal?ponin andα?SMA was negative, and a few SM?MHC proteins were expressed when PMVECs were incubated with the serum of healthy volunteers; the expression of calponin, α?SMA and SM?MHC was positive when PMVECs were incubated with the serum of patients with obstructive jaundice. Compared with the serum of healthy volunteers, the expression of SM?MHC was significantly up?regulated when PMVECs were incubated with the serum of patients with obstructive jaundice (P<0.05). The expression of calponin, α?SMA and SM?MHC was significantly up?regulated at T2,3 compared with that at T1 , and at T3 compared with that at T2 when PMVECs were incubated with the serum of patients with obstructive jaundice ( P<0.05) . Conclusion The serum of patients with obstructive jaundice promotes myogenic differentiation of human PMVECs, which is probably one of the mechanisms underlying intrapulmonary microvascular dilatation.
